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Leonard Rome, Ph.D.

Work Email Address:
lrome@mednet.ucla.edu

Office Address:
Office
615 Charles E. Young Drive South
BSRB 350A
Los Angeles, CA 90095
UNITED STATES

Work Phone Number:
(310) 825-0789



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Department / Division Affiliations
Professor, Biological Chemistry
Associate Director and Governing Board, California NanoSystems Institute

Research Interest:

Engineering of a naturally occurring cellular structure

<p>We are using molecular engineering of a naturally occurring cellular structure called a vault to develop a flexible, targetable nano-scale capsule. Vaults are abundant cellular particles of unknown function found in nearly all eukaryotes (cells containing a nucleus). Cryo-electron microscopy combined with single particle reconstruction has provided overall dimensions of the vault at 42 x 75 nanometers (a nanometer is a millionth of a meter). These measurements indicate that the vault is larger in mass and size than many viruses. The overall structure of the intact vault is like a hollow barrel with two protruding caps and an indented waist with a very thin shell surrounding an internal cavity large enough to encompass several hundred proteins. Thus, the vault particle is a nanocapsule with incredible potential for compound encapsulation, protection, and delivery. Using a well-characterized insect virus into which a cloned gene can be easily inserted, it is possible to produce large quantities of a given protein in cultured insect cells. We have collaborated with a number of groups to use this system to produce large quantities of the major vault protein (MVP). Interestingly, the protein is able to self-assemble into vault-like particles. These MVP-only vaults are somewhat irregular, often containing distorted caps. However, we have demonstrated that co-production of all three vault proteins (MVP, TEP1 and VPARP) in insect cells results in self-assembly of particles that appear identical to naturally occurring vaults. </p><p> By using molecular genetic techniques to modify the gene encoding the major vault protein, vault proteins have been produced with chemically active peptides attached to their sequence. These modified proteins are incorporated into the inside of the vault particle without altering its basic structure. We propose to produce modified vault particles in order to test the concept that vaults can be bioengineered to allow their use in a wide variety of biological applications including drug delivery, biological sensors, enzyme delivery, controlled release, and eventually as parts for nano-electrical machines.</p>

Bio:

Leonard H. Rome is a cell biologist and biochemist who has served on the UCLA School of Medicine faculty since he joined the Department of Biological Chemistry in 1979. He became a full professor in 1988 and wAs Senior Associate Dean for Research in the School of Medicine from 1997 - 2012. Dr. Rome earned his B.S. in Chemistry and M.S. and Ph.D. in Biological Chemistry at the University of Michigan, Ann Arbor. He was a postdoctoral fellow at the National Institutes of Health, where he worked on lysosome biogenesis. Dr. Rome has chaired the School of Medicine Faculty Executive Committee and is actively involved in graduate and medical education. He is a recipient of the School of Medicine Award for Excellence in Education. While Senior Associate Dean for Research, he organized a strategic plan for research in the School and spearheaded campus-wide efforts in genomics, proteomics, and computational biology. His laboratory research centers on a novel cellular organelle called a "vault" which was discovered in his laboratory. Dr. Rome is presently organizing a Nanoscience Interdisciplinnary Research Team, a collaboration of disciplines including cell biologists, engineers, chemists, and structural biologists who are engineering vaults so that they can be used in drug delivery.

Publications:

Matsumoto Nicholas M, Prabhakaran Panchami, Rome Leonard H, Maynard Heather D Smart vaults: thermally-responsive protein nanocapsules. ACS nano. 2013; 7(1): 867-74.
Rome Leonard H, Kickhoefer Valerie A Development of the Vault Particle as a Platform Technology. ACS nano. 2012; 7(1): .
Kar Upendra K, Jiang Janina, Champion Cheryl I, Salehi Sahar, Srivastava Minu, Sharma Sherven, Rabizadeh Shahrooz, Niazi Kayvan, Kickhoefer Valerie, Rome Leonard H, Kelly Kathleen A Vault nanocapsules as adjuvants favor cell-mediated over antibody-mediated immune responses following immunization of mice. PloS one. 2012; 7(7): e38553.
Yang Jian, Srinivasan Aswin, Sun Yang, Mrazek Jan, Shu Zhanyong, Kickhoefer Valerie A, Rome Leonard H Vault nanoparticles engineered with the protein transduction domain, TAT48, enhances cellular uptake. Integrative biology : quantitative biosciences from nano to macro. 2012; .
Upendra K. Kar, Minu K. Srivastava, Åsa Andersson, Felicita Baratelli, Min Huang, Valerie A. Kickhoefer, Steven M. Dubinett, Leonard H. Rome, Sherven Sharma Novel CCL21-Vault Nanocapsule Intratumoral Delivery Inhibits Lung Cancer Growth. PLoS One. 2011; 6(5): e18758.
Kar Upendra K, Srivastava Minu K, Andersson Asa, Baratelli Felicita, Huang Min, Kickhoefer Valerie A, Dubinett Steven M, Rome Leonard H, Sharma Sherven Novel CCL21-vault nanocapsule intratumoral delivery inhibits lung cancer growth. PloS one. 2011; 6(5): e18758.
Han Muri, Kickhoefer Valerie A, Nemerow Glen R, Rome Leonard H Targeted vault nanoparticles engineered with an endosomolytic peptide deliver biomolecules to the cytoplasm. ACS nano. 2011; 5(8): 6128-37.
Daniel C. Buehler, Daniel B. Toso, Valerie A. Kickhoefer, Z. Hong Zhou, Leonard H. Rome Vaults Engineered for Hydrophobic Drug Delivery. Small. 2011; Epub ahead of print: .
Buehler Daniel C, Toso Daniel B, Kickhoefer Valerie A, Zhou Z Hong, Rome Leonard H Vaults engineered for hydrophobic drug delivery. Small (Weinheim an der Bergstrasse, Germany). 2011; 7(10): 1432-9.
Xia Y, Ramgopal Y, Li H, Shang L, Srinivas P, Kickhoefer VA, Rome LH, Preiser PR, Boey F, Zhang H, Venkatraman SS Immobilization of Recombinant Vault Nanoparticles on Solid Substrates. ACS Nano. 2010; 4(3): 1417-24.
Xia Yun, Ramgopal Yamini, Li Hai, Shang Lei, Srinivas Parisa, Kickhoefer Valerie A, Rome Leonard H, Preiser Peter R, Boey Freddy, Zhang Hua, Venkatraman Subbu S Immobilization of recombinant vault nanoparticles on solid substrates. ACS nano. 2010; 4(3): 1417-24.
Yang J, Kickhoefer VA, Ng BC, Gopal A, Bentolila LA, John S, Tolbert SH, Rome LH Vaults are dynamically unconstrained cytoplasmic nanoparticles capable of half vault exchange. ACS Nano. 2010; 4(12): 7229-40.
Yang Jian, Kickhoefer Valerie A, Ng Benny C, Gopal Ajaykumar, Bentolila Laurent A, John Scott, Tolbert Sarah H, Rome Leonard H Vaults are dynamically unconstrained cytoplasmic nanoparticles capable of half vault exchange. ACS nano. 2010; 4(12): 7229-40.
Champion Cheryl I, Kickhoefer Valerie A, Liu Guangchao, Moniz Raymond J, Freed Amanda S, Bergmann Liisa L, Vaccari Dana, Raval-Fernandes Sujna, Chan Ann M, Rome Leonard H, Kelly Kathleen A A vault nanoparticle vaccine induces protective mucosal immunity. PloS one. 2009; 4(4): e5409.
Esfandiary, R., Kickhoefer, V.A., Rome, L.H., Joshi, S.B., Middaugh, C.R. Structural stability of vault particles. Journal of Pharmaceutical Sciences. 2009; 98(4): 1376-86.
Kickhoefer, V. A., Han, M., Raval-Fernandes, S., Poderycki, M. J., Moniz, R. J., Vaccari, D., Silvestry, M., Stewart, P. L., Kelly, K. A., Rome, L. H. Targeting vault nanoparticles to specific cell surface receptors. ACS Nano. 2009; 3(1): 27-36.
Kickhoefer Valerie A, Han Muri, Raval-Fernandes Sujna, Poderycki Michael J, Moniz Raymond J, Vaccari Dana, Silvestry Mariena, Stewart Phoebe L, Kelly Kathleen A, Rome Leonard H Targeting vault nanoparticles to specific cell surface receptors. ACS nano. 2009; 3(1): 27-36.
Goldsmith LE, Pupols M, Kickhoefer VA, Rome LH, Monbouquette HG Utilization of a protein "shuttle" to load vault nanocapsules with gold probes and proteins. ACS Nano. 2009; 3(10): 3175-83.
Goldsmith Lisa E, Pupols Melody, Kickhoefer Valerie A, Rome Leonard H, Monbouquette Harold G Utilization of a protein "shuttle" to load vault nanocapsules with gold probes and proteins. ACS nano. 2009; 3(10): 3175-83.
Lai CY, Wiethoff CM, Kickhoefer VA, Rome LH, Nemerow GR Vault nanoparticles containing an adenovirus-derived membrane lytic protein facilitate toxin and gene transfer. ACS Nano. 2009; 3(3): 691-9.
Lai CY, Wiethoff CM, Kickhoefer VA, Rome LH, Nemerow GR Vault nanoparticles containing an adenovirus-derived membrane lytic protein facilitate toxin and gene transfer. ACS Nano. 2009; 3(3): 691-9.
Lai Cheng-Yu, Wiethoff Chris M, Kickhoefer Valerie A, Rome Leonard H, Nemerow Glen R Vault nanoparticles containing an adenovirus-derived membrane lytic protein facilitate toxin and gene transfer. ACS nano. 2009; 3(3): 691-9.
Ng, B.C., Yu, M., Gopal, A., Rome, L.H., Monbouquette, H.G., Tolbert, S.H. Encapsulation of semiconducting polymers in vault protein cages. Nano Letters. 2008; 8(10): 3503-9.
Xia, T., Rome, L.H., Nel, A. Nanobiology: Particles slip cell security. Nature Materials. 2008; 7(7): 519-20.
Yu, M., Ng, B.C., Rome, L.H., Tolbert, S.H., Monbouquette, H.G. Reversible pH lability of cross-linked vault nanocapsules. Nano Letters. 2008; 8(10): 3510-5.
Anderson, D.H., Kickhoefer, V.A., Sievers, S.A., Rome, L.H., Eisenberg, D. Draft crystal structure of the vault shell at 9-A resolution. PLoS biology. 2007; 5(11): e318.
Kaddis, C.S., Lomeli, S.H., Yin, S., Berhane, B., Apostol, M.I., Kickhoefer, V.A., Rome, L.H., Loo, J.A. Sizing large proteins and protein complexes by electrospray ionization mass spectrometry and ion mobility. Journal of the American Society for Mass Spectrometry. 2007; 18(7): 1206-16.
Goldsmith, L.E., Yu, M., Rome, L.H., Monbouquette, H.G. Vault nanocapsule dissociation into halves triggered at low pH. Biochemistry. 2007; 46(10): 2865-75.
Slesina, M., Inman, E.M., Moore, A.E., Goldhaber, J.I., Rome, L.H., Volknandt, W. Movement of vault particles visualized by GFP-tagged major vault protein. Cell and Tissue Research. 2006; 324(3): 403-10.
Poderycki, M.J., Kickhoefer, V.A., Kaddis, C.S., Raval-Fernandes, S., Johansson, E., Zink, J.I., Loo, J.A., Rome, L.H. The vault exterior shell is a dynamic structure that allows incorporation of vault-associated proteins into its interior. Biochemistry. 2006; 45(39): 12184-93.
Kickhoefer, V.A., Garcia, Y., Mikyas, Y., Johansson, E., Zhou, J.C., Raval-Fernandes, S., Minoofar, P., Zink, J.I., Dunn, B., Stewart, P.L., Rome, L.H. Engineering of vault nanocapsules with enzymatic and fluorescent properties. Proceedings of the National Academy of Sciences of the United States of America. 2005; 102(12): 4348-52.
Raval-Fernandes, S., Kickhoefer, V.A., Kitchen, C., Rome, L.H. Increased susceptibility of vault poly(ADP-ribose) polymerase-deficient mice to carcinogen-induced tumorigenesis. Cancer Research. 2005; 65(19): 8846-52.
Slesina, M., Inman, E.M., Rome, L.H., Volknandt, W. Nuclear localization of the major vault protein in U373 cells. Cell and Tissue Research. 2005; 321(1): 97-104.
Poderycki, M.J., Rome, L.H., Harrington, L., Kickhoefer, V.A. The p80 homology region of TEP1 is sufficient for its association with the telomerase and vault RNAs, and the vault particle. Nucleic Acids Research. 2005; 33(3): 893-902.
Emre, N., Raval-Fernandes, S., Kickhoefer, V.A., Rome, L.H. Analysis of MVP and VPARP promoters indicates a role for chromatin remodeling in the regulation of MVP. Biochimica et Biophysica Acta. 2004; 1678(1): 33-46.
Mikyas, Y., Makabi, M., Raval-Fernandes, S., Harrington, L., Kickhoefer, V.A., Rome, L.H., Stewart, P.L. Cryoelectron microscopy imaging of recombinant and tissue derived vaults: localization of the MVP N termini and VPARP. Journal of Molecular Biology. 2004; 344(1): 91-105.
Liu, Y., Snow, B.E., Kickhoefer, V.A., Erdmann, N., Zhou, W., Wakeham, A., Gomez, M., Rome, L.H., Harrington, L. Vault poly(ADP-ribose) polymerase is associated with mammalian telomerase and is dispensable for telomerase function and vault structure in vivo. Molecular and Cellular Biology. 2004; 24(12): 5314-23.
Kickhoefer, V.A., Emre, N., Stephen, A.G., Poderycki, M.J., Rome, L.H. Identification of conserved vault RNA expression elements and a non-expressed mouse vault RNA gene. Gene. 2003; 309(2): 65-70.
Hu, Y., Stephen, A.G., Cao, J., Tanzer, L.R., Slapak, C.A., Harrison, S.D., Devanarayan, V., Dantzig, A.H., Starling, J.J., Rome, L.H., Moore, R.E. A very early induction of major vault protein accompanied by increased drug resistance in U-937 cells. International journal of cancer. 2002; 97(2): 149-56.
Kickhoefer, V.A., Poderycki, M.J., Chan, E.K.L., Rome, L.H. The La RNA-binding protein interacts with the vault RNA and is a vault-associated protein. The Journal of Biological Chemistry. 2002; 277(43): 41282-6.
Stephen, A. G., Raval-Fernandes, S., Huynh, T., Torres, M., Kickhoefer, V. A., Rome, L. H. Assembly of vault-like particles in insect cells expressing only the major vault protein. J Biol Chem. 2001; 276(26): 23217-20.
Stephen A G, Raval-Fernandes S, Huynh T, Torres M, Kickhoefer V A, Rome L H Assembly of vault-like particles in insect cells expressing only the major vault protein. The Journal of biological chemistry. 2001; 276(26): 23217-20.
Kickhoefer, V. A., Liu, Y., Kong, L. B., Snow, B. E., Stewart, P. L., Harrington, L., Rome, L. H. The Telomerase/vault-associated protein TEP1 is required for vault RNA stability and its association with the vault particle. J Cell Biol. 2001; 152(1): 157-64.
Kickhoefer V A, Liu Y, Kong L B, Snow B E, Stewart P L, Harrington L, Rome L H The Telomerase/vault-associated protein TEP1 is required for vault RNA stability and its association with the vault particle. The Journal of cell biology. 2001; 152(1): 157-64.
Siva, A. C., Raval-Fernandes, S., Stephen, A. G., LaFemina, M. J., Scheper, R. J., Kickhoefer, V. A., Rome, L. H. Up-regulation of vaults may be necessary but not sufficient for multidrug resistance. Int J Cancer. 2001; 92(2): 195-202.
Siva A C, Raval-Fernandes S, Stephen A G, LaFemina M J, Scheper R J, Kickhoefer V A, Rome L H Up-regulation of vaults may be necessary but not sufficient for multidrug resistance. International journal of cancer. Journal international du cancer. 2001; 92(2): 195-202.
Marbois, B. N., Faull, K. F., Fluharty, A. L., Raval-Fernandes, S., Rome, L. H. Analysis of sulfatide from rat cerebellum and multiple sclerosis white matter by negative ion electrospray mass spectrometry. Biochim Biophys Acta. 2000; 1484(1): 59-70.
Marbois B N, Faull K F, Fluharty A L, Raval-Fernandes S, Rome L H Analysis of sulfatide from rat cerebellum and multiple sclerosis white matter by negative ion electrospray mass spectrometry. Biochimica et biophysica acta. 2000; 1484(1): 59-70.
Kong, L. B., Siva, A. C., Kickhoefer, V. A., Rome, L. H., Stewart, P. L. RNA location and modeling of a WD40 repeat domain within the vault. . 2000; 6(6): 890-900.
Kong L B, Siva A C, Kickhoefer V A, Rome L H, Stewart P L RNA location and modeling of a WD40 repeat domain within the vault. RNA (New York, N.Y.). 2000; 6(6): 890-900.
Schroeijers A B, Siva A C, Scheffer G L, de Jong M C, Bolick S C, Dukers D F, Slootstra J W, Meloen R H, Wiemer E, Kickhoefer V A, Rome L H, Scheper R J The Mr 193,000 vault protein is up-regulated in multidrug-resistant cancer cell lines. Cancer research. 2000; 60(4): 1104-10.
Raval-Fernandes S, Kickhoefer V A, Rome L H Cloning of a cDNA encoding a sequence-specific single-stranded-DNA-binding protein from Rattus norvegicus. Gene. 1999; 237(1): 201-7.
Kong L B, Siva A C, Rome L H, Stewart P L Structure of the vault, a ubiquitous celular component. Structure (London, England : 1993). 1999; 7(4): 371-9.
Kickhoefer V A, Siva A C, Kedersha N L, Inman E M, Ruland C, Streuli M, Rome L H The 193-kD vault protein, VPARP, is a novel poly(ADP-ribose) polymerase. The Journal of cell biology. 1999; 146(5): 917-28.
Kickhoefer V A, Stephen A G, Harrington L, Robinson M O, Rome L H Vaults and telomerase share a common subunit, TEP1. The Journal of biological chemistry. 1999; 274(46): 32712-7.
Raval-Fernandes S, Rome L H Role of axonal components during myelination. Microscopy research and technique. 1998; 41(5): 379-92.
Kickhoefer V A, Rajavel K S, Scheffer G L, Dalton W S, Scheper R J, Rome L H Vaults are up-regulated in multidrug-resistant cancer cell lines. The Journal of biological chemistry. 1998; 273(15): 8971-4.
Raval-Fernandes S, Sawant L A, Aebersold R H, Ducret A, Rome L H Axonal proteins involved in myelination: characterization of a collagen-like protein. Developmental neuroscience. 1997; 19(5): 421-9.
Kickhoefer V A, Vasu S K, Rome L H Vaults are the answer, what is the question?. Trends in cell biology. 1996; 6(5): 174-8.
Malek-Hedayat S, Rome L H Cloning and sequence of the cDNA encoding the rat oligodendrocyte integrin beta 1 subunit. Gene. 1995; 158(2): 287-90.
Vasu S K, Rome L H Dictyostelium vaults: disruption of the major proteins reveals growth and morphological defects and uncovers a new associated protein. The Journal of biological chemistry. 1995; 270(28): 16588-94.
Rome L H Multidrug resistance: locked in the vault?. Nature medicine. 1995; 1(6): 527.
Notterpek L M, Rome L H A protein involved in central nervous system myelination: localization in the extracellular matrix and induction in neuroblastoma cells. Developmental neuroscience. 1994; 16(5-6): 267-78.
Malek-Hedayat S, Rome L H Expression of a beta 1-related integrin by oligodendroglia in primary culture: evidence for a functional role in myelination. The Journal of cell biology. 1994; 124(6): 1039-46.
Notterpek L M, Rome L H Functional evidence for the role of axolemma in CNS myelination. Neuron. 1994; 13(2): 473-85.
Hamilton S P, Rome L H Stimulation of in vitro myelin synthesis by microglia. Glia. 1994; 11(4): 326-35.
Vilalta A, Kickhoefer V A, Rome L H, Johnson D L The rat vault RNA gene contains a unique RNA polymerase III promoter composed of both external and internal elements that function synergistically. The Journal of biological chemistry. 1994; 269(47): 29752-9.
Kickhoefer V A, Rome L H The sequence of a cDNA encoding the major vault protein from Rattus norvegicus. Gene. 1994; 151(1-2): 257-60.
Chugani D C, Rome L H, Kedersha N L Evidence that vault ribonucleoprotein particles localize to the nuclear pore complex. Journal of cell science. 1993; 106 ( Pt 1)(6): 23-9.
Notterpek L M, Bullock P N, Malek-Hedayat S, Fisher R, Rome L H Myelination in cerebellar slice cultures: development of a system amenable to biochemical analysis. Journal of neuroscience research. 1993; 36(6): 621-34.
Grody W W, Kronquist K E, Lee E U, Edmond J, Rome L H PCR-based cystic fibrosis (CF) carrier screening in a first-year medical student biochemistry laboratory. American journal of human genetics. 1993; 53(6): 1352-5.
Kickhoefer V A, Searles R P, Kedersha N L, Garber M E, Johnson D L, Rome L H Vault ribonucleoprotein particles from rat and bullfrog contain a related small RNA that is transcribed by RNA polymerase III. The Journal of biological chemistry. 1993; 268(11): 7868-73.
Vasu S K, Kedersha N L, Rome L H cDNA cloning and disruption of the major vault protein alpha gene (mvpA) in Dictyostelium discoideum. The Journal of biological chemistry. 1993; 268(21): 15356-60.
Malek-Hedayat S, Rome L H Expression of multiple integrins and extracellular matrix components by C6 glioma cells. Journal of neuroscience research. 1992; 31(3): 470-8.
Chugani D C, Kedersha N L, Rome L H Vault immunofluorescence in the brain: new insights regarding the origin of microglia. The Journal of neuroscience : the official journal of the Society for Neuroscience. 1991; 11(1): 256-68.
Kedersha N L, Heuser J E, Chugani D C, Rome L H Vaults. III. Vault ribonucleoprotein particles open into flower-like structures with octagonal symmetry. The Journal of cell biology. 1991; 112(2): 225-35.
Bullock P N, Rome L H Glass micro-fibers: a model system for study of early events in myelination. Journal of neuroscience research. 1990; 27(3): 383-93.
Rome L H, Cardwell M C, Bullock P N, Hamilton S P Matrix interactions regulating myelinogenesis in cultured oligodendrocytes. Advances in experimental medicine and biology. 1990; 265(4): 157-67.
Kedersha N L, Miquel M C, Bittner D, Rome L H Vaults. II. Ribonucleoprotein structures are highly conserved among higher and lower eukaryotes. The Journal of cell biology. 1990; 110(4): 895-901.
Kedersha N L, Rome L H Vaults: large cytoplasmic RNP's that associate with cytoskeletal elements. Molecular biology reports. 1990; 14(2-3): 121-2.
Westcott K R, Rome L H Cation-independent mannose 6-phosphate receptor contains covalently bound fatty acid. Journal of cellular biochemistry. 1988; 38(1): 23-33.
Cardwell M C, Rome L H Evidence that an RGD-dependent receptor mediates the binding of oligodendrocytes to a novel ligand in a glial-derived matrix. The Journal of cell biology. 1988; 107(4): 1541-9.
Cardwell M C, Rome L H RGD-containing peptides inhibit the synthesis of myelin-like membrane by cultured oligodendrocytes. The Journal of cell biology. 1988; 107(4): 1551-9.
Bame K J, Rome L H Acetyl-CoA: alpha-glucosaminide N-acetyltransferase from rat liver. Methods in enzymology. 1987; 138(13): 607-11.
Westcott K R, Searles R P, Rome L H Evidence for ligand- and pH-dependent conformational changes in liposome-associated mannose 6-phosphate receptor. The Journal of biological chemistry. 1987; 262(13): 6101-7.
Lemansky P, Hasilik A, von Figura K, Helmy S, Fishman J, Fine R E, Kedersha N L, Rome L H Lysosomal enzyme precursors in coated vesicles derived from the exocytic and endocytic pathways. The Journal of cell biology. 1987; 104(6): 1743-8.
Bame K J, Rome L H Acetyl-coenzyme A:alpha-glucosaminide N-acetyltransferase. Evidence for an active site histidine residue. The Journal of biological chemistry. 1986; 261(22): 10127-32.
Bame K J, Rome L H Genetic evidence for transmembrane acetylation by lysosomes. Science (New York, N.Y.). 1986; 233(4768): 1087-9.
Tietze F, Rome L H, Butler J D, Harper G S, Gahl W A Impaired clearance of free cystine from lysosome-enriched granular fractions of I-cell-disease fibroblasts. The Biochemical journal. 1986; 237(1): 9-15.
Kedersha N L, Rome L H Isolation and characterization of a novel ribonucleoprotein particle: large structures contain a single species of small RNA. The Journal of cell biology. 1986; 103(3): 699-709.
Rome L H, Hill D F Lysosomal degradation of glycoproteins and glycosaminoglycans. Efflux and recycling of sulphate and N-acetylhexosamines. The Biochemical journal. 1986; 235(3): 707-13.
Kedersha N L, Rome L H Preparative agarose gel electrophoresis for the purification of small organelles and particles. Analytical biochemistry. 1986; 156(1): 161-70.
Kedersha N L, Hill D F, Kronquist K E, Rome L H Subpopulations of liver coated vesicles resolved by preparative agarose gel electrophoresis. The Journal of cell biology. 1986; 103(1): 287-97.
Rome L H, Bullock P N, Chiappelli F, Cardwell M, Adinolfi A M, Swanson D Synthesis of a myelin-like membrane by oligodendrocytes in culture. Journal of neuroscience research. 1986; 15(1): 49-65.
Bame K J, Rome L H Acetyl coenzyme A: alpha-glucosaminide N-acetyltransferase. Evidence for a transmembrane acetylation mechanism. The Journal of biological chemistry. 1985; 260(20): 11293-9.
Hill D F, Bullock P N, Chiappelli F, Rome L H Binding and internalization of lysosomal enzymes by primary cultures of rat glia. Journal of neuroscience research. 1985; 14(1): 35-47.
Mehrabian M, Bame K J, Rome L H Interaction of rat liver lysosomal membranes with actin. The Journal of cell biology. 1984; 99(2): 680-5.
Berg R A, Schwartz M L, Rome L H, Crystal R G Lysosomal function in the degradation of defective collagen in cultured lung fibroblasts. Biochemistry. 1984; 23(10): 2134-8.
Campbell C H, Fine R E, Squicciarini J, Rome L H Coated vesicles from rat liver and calf brain contain cryptic mannose 6-phosphate receptors. The Journal of biological chemistry. 1983; 258(4): 2628-33.
Campbell C H, Rome L H Coated vesicles from rat liver and calf brain contain lysosomal enzymes bound to mannose 6-phosphate receptors. The Journal of biological chemistry. 1983; 258(21): 13347-52.
Campbell C H, Miller A L, Rome L H Incorporation of mannose 6-phosphate receptors into liposomes. Receptor topography and binding of alpha-mannosidase. The Biochemical journal. 1983; 214(2): 413-9.
Rome L H, Hill D F, Bame K J, Crain L R Utilization of exogenously added acetyl coenzyme A by intact isolated lysosomes. The Journal of biological chemistry. 1983; 258(5): 3006-11.
Steiner A W, Rome L H Assay and purification of a solubilized membrane receptor that binds the lysosomal enzyme alpha-L-iduronidase. Archives of biochemistry and biophysics. 1982; 214(2): 681-7.
Rome L H Uptake and binding of alpha-L-iduronidase. Methods in enzymology. 1982; 83(2): 582-7.
Rome L H alpha-L-iduronidase from human kidney. Methods in enzymology. 1982; 83(2): 578-82.
Rome L H, Crain L R Degradation of mucopolysaccharide in intact isolated lysosomes. The Journal of biological chemistry. 1981; 256(21): 10763-8.
Rome L H, Miller J Butanedione treatment reduces receptor binding of a lysosomal enzyme to cells and membranes. Biochemical and biophysical research communications. 1980; 92(3): 986-93.
Rome L H, Weissmann B, Neufeld E F Direct demonstration of binding of a lysosomal enzyme, alpha-L-iduronidase, to receptors on cultured fibroblasts. Proceedings of the National Academy of Sciences of the United States of America. 1979; 76(5): 2331-4.
Rome L H, Garvin A J, Allietta M M, Neufeld E F Two species of lysosomal organelles in cultured human fibroblasts. Cell. 1979; 17(1): 143-53.
Rome L H, Garvin A J, Neufeld E F Human kidney alpha-L-iduronidase: purification and characterization. Archives of biochemistry and biophysics. 1978; 189(2): 344-53.
Neufeld E F, Sando G N, Garvin A J, Rome L H The transport of lysosomal enzymes. Journal of supramolecular structure. 1977; 6(1): 95-101.
Rome L H, Lands W E, Roth G J, Majerus P W Aspirin as a quantitative acetylating reagent for the fatty acid oxygenase that forms prostaglandins. Prostaglandins. 1976; 11(1): 23-30.
Lands W E, Cook H W, Rome L H Prostaglandin biosynthesis: consequences of oxygenase mechanism upon in vitro assays of drug effectiveness. Advances in prostaglandin and thromboxane research. 1976; 1(1): 7-17.