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Enrico Stefani, M.D., Ph.D.

Email Address:
estefani@ucla.edu

Work Address:
Laboratory
CHS
Los Angeles, CA 90095
UNITED STATES

Office
CHS
Box 957115
Los Angeles, CA 90095
UNITED STATES

Work Phone Number:
(310) 794-7808
310-794-7804



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Department / Division Affiliations
Professor, Anesthesiology
Member, ACCESS Program: Dept. of Molecular, Cell & Integrative Physiology, Molecular Medicine, NanoBiotechnology and Biomaterials, Brain Research Institute, California NanoSystems Institute, Neuroscience IDP
Faculty, Cardiovascular Research Laboratory, Molecular, Cellular, and Integrative Physiology IDP

Bio:

Dr. Stefani obtained his MD at the University of Buenos Aires, Argentine and his PhD in Physiology at the Department of Biophysics, University College London under the direction of Dr. R. Miledi FRS and Dr. Sir Bernard Katz, FRS. Dr. Stefani is distinguished by opening new research fields with technical innovations using biophysical approaches. He discovered neuronal serotonin receptors, nerve trophic influence on channel expression, L-type calcium channels in skeletal muscle, and hormonal regulation of ion channels and mechanisms in heart hypertrophy. He pioneered molecular aspects K and Ca channels voltage-sensing mechanisms. Dr Stefani discovered that L-type calcium channels of skeletal muscle as nifedipine receptors can have a role on excitation-contraction coupling. He pioneered studies on voltage dependent ion channel excitability with high resolution recordings in expressed K and Ca channels. He recorded the electrical manifestation of voltage dependent conformation al changes of ion channels referred as gating currents and demonstrated that these precedes pore opening that is also regulated by beta subunits. Recently Dr. Stefani is investigating mechanisms of heart protection during ischemia developing high resolution microscopy techniques beyond the diffraction limit. Dr. Stefani has more than 200 publications in frontier journals

Publications:

Martin Lisa J, Lau Edward, Singh Harpreet, Vergnes Laurent, Tarling Elizabeth J, Mehrabian Margarete, Mungrue Imran, Xiao Sheila, Shih Diana, Castellani Larry, Ping Peipei, Reue Karen, Stefani Enrico, Drake Thomas A, Bostrom Kristina, Lusis Aldons J ABCC6 Localizes to the Mitochondria-Associated Membrane. Circulation research. 2012; 586(16): .
Wu Yong, Zinchuk Vadim, Grossenbacher-Zinchuk Olga, Stefani Enrico Critical evaluation of quantitative colocalization analysis in confocal fluorescence microscopy. Interdisciplinary sciences, computational life sciences. 2012; 4(1): 27-37.
Scruggs Sarah B, Zong Nobel C, Wang Ding, Stefani Enrico, Ping Peipei Post-translational modification of cardiac proteasomes: functional delineation enabled by proteomics. American journal of physiology. Heart and circulatory physiology. 2012; 303(1): H9-H18.
Morera Francisco J, Alioua Abderrahmane, Kundu Pallob, Salazar Marcelo, Gonzalez Carlos, Martinez Agustin D, Stefani Enrico, Toro Ligia, Latorre Ramon The first transmembrane domain (TM1) of β2-subunit binds to the transmembrane domain S1 of α-subunit in BK potassium channels. FEBS letters. 2012; 586(16): 2287-93.
Singh Harpreet, Lu Rong, Rodríguez Pedro Felipe Gardeazábal, Wu Yong, Bopassa Jean Chrisostome, Stefani Enrico, Toro Ligia Visualization and quantification of cardiac mitochondrial protein clusters with STED microscopy. Mitochondrion. 2012; 12(2): 230-6.
Danesh Shahab M, Kundu Pallob, Lu Rong, Stefani Enrico, Toro Ligia Distinct transcriptional regulation of human large conductance voltage- and calcium-activated K+ channel gene (hSlo1) by activated estrogen receptor alpha and c-Src tyrosine kinase. The Journal of biological chemistry. 2011; 286(36): 31064-71.
Zinchuk Vadim, Wu Yong, Grossenbacher-Zinchuk Olga, Stefani Enrico Quantifying spatial correlations of fluorescent markers using enhanced background reduction with protein proximity index and correlation coefficient estimations. Nature protocols. 2011; 6(10): 1554-67.
Alioua Abderrahmane, Li Min, Wu Yong, Stefani Enrico, Toro Ligia Unconventional myristoylation of large-conductance Ca²⁺-activated K⁺ channel (Slo1) via serine/threonine residues regulates channel surface expression. Proceedings of the National Academy of Sciences of the United States of America. 2011; 108(26): 10744-9.
Bopassa JC, Eghbali M, Toro L, Stefani E A novel estrogen receptor GPER inhibits mitochondria permeability transition pore opening and protects the heart against ischemia-reperfusion injury. Am J Physiol Heart Circ Physiol. 2010; 298(1): H16-23.
Wu Yong, Eghbali Mansoureh, Ou Jimmy, Lu Rong, Toro Ligia, Stefani Enrico Quantitative determination of spatial protein-protein correlations in fluorescence confocal microscopy. Biophysical journal. 2010; 98(3): 493-504.
Zhang Michael J, Franklin Sarah, Li Yifeng, Wang Sujing, Ru Xiaochen, Mitchell-Jordan Scherise A, Mano Hiroyuki, Stefani Enrico, Ping Peipei, Vondriska Thomas M Stress signaling by Tec tyrosine kinase in the ischemic myocardium. American journal of physiology. Heart and circulatory physiology. 2010; 299(3): H713-22.
Li Min, Tanaka Yoshio, Alioua Abderrahmane, Wu Yong, Lu Rong, Kundu Pallob, Sanchez-Pastor Enrique, Marijic Jure, Stefani Enrico, Toro Ligia Thromboxane A2 receptor and MaxiK-channel intimate interaction supports channel trans-inhibition independent of G-protein activation. Proceedings of the National Academy of Sciences of the United States of America. 2010; 107(44): 19096-101.
Helguera, G. Eghbali, M. Sforza, D. Minosyan, T. Y. Toro, L. Stefani, E. Changes in global gene expression in rat myometrium in transition from late pregnancy to parturition. Physiological Genomics. 2009; 36(2): 89-97.
Vlashi, E. Kim, K. Lagadec, C. Donna, L. D. McDonald, J. T. Eghbali, M. Sayre, J. W. Stefani, E. McBride, W. Pajonk, F. In Vivo Imaging, Tracking, and Targeting of Cancer Stem Cells. Journal of the National Cancer Institute. 2009; 101(5): 350-359.
Stefani, E. Boffetta, P. Ronco, A. Deneo-Pellegrini, H. Acosta, G. Mendilaharsu, M. Dietary patterns and risk of bladder cancer: a factor analysis in Uruguay. Cancer Causes & Control. 2008; 19(10): 1243-1249.
Gomes, P. Saito, T. del Corsso, C. Alioua, A. Eghbali, M. Toro, L. Stefani, E. Identification of a functional interaction between Kv4.3 channels and c-Src tyrosine kinase. Biochimica Et Biophysica Acta-Molecular Cell Research. 2008; 1783(10): 1884-1892.
Alioua, A. Lu, R. Kumar, Y. Eghbali, M. Kundu, P. Toro, L. Stefani, E. Slo1 caveolin-binding motif, a mechanism of caveolin-1-Slo1 interaction regulating Slo1 surface expression. Journal of Biological Chemistry. 2008; 283(8): 4808-4817.
Kundu, P. Alioua, A. Stefani, E. Toro, L. A genomic mechanism of estrogen action regulates mouse MaxiK alpha subunit expression from its proximal promoter. Biophysical Journal 2007; 94a-94a.
Minosyan, T. Y. Ciobotaru, A. Toro, L. Stefani, E. Eghbali, M. Caveolin 3 associates with KCNE2 and ERG1 in adult mouse heart. Biophysical Journal. 2007; 126a-126a.
Fishfader, P. Minosyan, T. Y. Eghbali, M. Toro, L. Stefani, E. Distinct regulation of estrogen receptor alpha isoforms by estrogen. Biophysical Journal 2007; 620a-620a.
Zarei, M. M. Song, M. Wilson, R. J. Cox, N. Colom, L. V. Knaus, H. G. Stefani, E. Toro, L. Endocytic trafficking signals in KCNMB2 regulate surface expression of a large conductance voltage and Ca(2+)-activated K+ channel. Neuroscience. 2007; 147(1): 80-9.
Saito, T. Eghbali, M. Vallejo, J. Toro, L. Stefani, E. Gender regulates the transient outward K+ currents in mouse right ventricular myocytes. Biophysical Journal 2007; 463a-463a.
Minosyan, T. Y. Lu, R. Eghbali, M. Toro, L. Stefani, E. Increased 5-HT contractile response in late pregnant rat myometrium may result from a higher density of 5-HT2A receptors. Biophysical Journal. 2007; volume(number): 132a-132a.
Minosyan, T. Y. Lu, R. Eghbali, M. Toro, L. Stefani, E. Increased 5-HT contractile response in late pregnant rat myometrium is associated with a higher density of 5-HT2A receptors. Journal of Physiology-London. 2007; 581(1): 91-97.
Cavalli, A. Eghbali, M. Minosyan, T. Y. Stefani, E. Philipson, K. D. Localization of sarcolemmal proteins to lipid rafts in the myocardium. Cell Calcium. 2007; 42(3): 313-22.
Kundu, P. Alioua, A. Stefani, E. Toro, L. Regulation of Mouse Slo Gene Expression - Multiple promoters, transcription start sites, and genomic action of estrogen. Journal of Biological Chemistry. 2007; 282(37): 27478-27492.
Lu, R. Alioua, A. Kumar, Y. Stefani, E. Toro, L. Tyrosine phosphorylation motif "pYxx psi" in 5-HT2AR is dispensable for its interaction with c-Src in vitro. Biophysical Journal 2007; 433a-433a.
Vallejo, J. Eghbali, M. Toro, L. Stefani, E. Altered trafficking of Large Conductance Ca2+-activated K+ (MaxiK) channels in mouse myometrium during late pregnancy. Faseb Journal. 2006; 20(5): A1241-A1242.
Ropero, A. B. Eghbali, M. Minosyan, T. Y. Tang, G. H. Toro, L. Stefani, E. Heart estrogen receptor alpha: Distinct membrane and nuclear distribution patterns and regulation by estrogen. Journal of Molecular and Cellular Cardiology. 2006; 41(3): 496-510.
Eghbali, M. Wang, Y. B. Toro, L. Stefani, E. Heart hypertrophy during pregnancy: A better functioning heart?. Trends in Cardiovascular Medicine. 2006; 16(8): 285-291.
Toro, B. Cox, N. Wilson, R. J. Garrido-Sanabria, E. Stefani, E. Toro, L. Zarei, M. M. KCNMB1 regulates surface expression of a voltage and Ca2+-activated K+ channel via endocytic trafficking signals. Neuroscience. 2006; 142(3): 661-9.
Tanaka, Y. Tang, G. Takizawa, K. Otsuka, K. Eghbali, M. Song, M. Nishimaru, K. Shigenobu, K. Koike, K. Stefani, E. Toro, L. Kv channels contribute to nitric oxide- and atrial natriuretic peptide-induced relaxation of a rat conduit artery. J Pharmacol Exp Ther. 2006; 317(1): 341-54.
Gomes, A. V. Zong, C. Edmondson, R. D. Li, X. Stefani, E. Zhang, J. Jones, R. C. Thyparambil, S. Wang, G. W. Qiao, X. Bardag-Gorce, F. Ping, P. P. Mapping the murine cardiac 26S proteasome complexes. Circulation Research. 2006; 99(4): 362-371.
Lu, R. Alioua, A. Kumar, Y. Eghbali, M. Stefani, E. Toro, L. MaxiK channel partners: physiological impact. Journal of Physiology-London. 2006; 570(1): 65-72.
Rueda, A. Song, M. Toro, L. Stefani, E. Valdivia, H. H. Sorcin modulation of Ca2+ sparks in rat vascular smooth muscle cells. J Physiol. 2006; 576(Pt 3): 887-901.
Lu, Gang Kang, Young Jun Han, Jiahuai Herschman, Harvey R. Stefani, Enrico Wang, Yibin TAB-1 modulates intracellular localization of p38 MAP kinase and downstream signaling. Journal of Biological Chemistry. 2006; 281(9): 6087-6095.
Ropero, A. B. Eghbali, M. Deva, R. Toro, L. Stefani, E. A 43 kDa form of estrogen receptor alpha (ER alpha) associates with membrane structures in rat male hearts. Steroids 2005; 70(5-7): 480-480.
Zhu, N. Eghbali, M. Helguera, G. Song, M. Stefani, E. Toro, L. Alternative splicing of Slo channel gene programmed by estrogen, progesterone and pregnancy. FEBS Lett. 2005; 579(21): 4856-60.
Eghbali, M. Deva, R. Alioua, A. Minosyan, T. Y. Ruan, H. M. Wang, Y. B. Toro, L. Stefani, E. Molecular and functional signature of heart hypertrophy during pregnancy. Circulation Research. 2005; 96(11): 1208-1216.
Zarei, M. M. Eghbali, M. Alioua, A. Song, M. Knaus, H. G. Stefani, E. Toro, L. An endoplasmic reticulum trafficking signal prevents surface expression of a voltage- and Ca2+-activated K+ channel splice variant. Proceedings of the National Academy of Sciences of the United States of America. 2004; 101(27): 10072-10077.
Tanaka, Y. Koike, K. Alioua, A. Shigenobu, K. Stefani, E. Toro, L. Beta1-subunit of MaxiK channel in smooth muscle: a key molecule which tunes muscle mechanical activity. J Pharmacol Sci. 2004; 94(4): 339-47.
Alioua, A. Eghbali, M. Minosyan, T. Stefani, E. Toro, L. Brain MaxiK channel, c-Src and caveolin-1 macromolecular complex: Lipid raft and microvessel localization. Biophysical Journal 2004; 86(1): 122a-122a.
Virgen, A. Peraza, A. L. Stefani, E. Toro, L. Muniz, J. Changes in cardiac tissue stiffness associated to functional hypertrophy during pregnancy. Biophysical Journal 2004; 86(1): 385a-385a.
Garcia-Valdes, J. Alioua, A. Eghbali, M. Stefani, E. Toro, L. Different pattern of expression and subcellular localization of c-Src, Yes, and Fyn in rat aortic myocytes. Biophysical Journal 2004; 86(1): 392a-393a.
Pagani, R. Song, M. McEnery, M. Qin, N. Tsien, R. W. Toro, L. Stefani, E. Uchitel, O. D. Differential expression of alpha 1 and beta subunits of voltage dependent Ca2+ channel at the neuromuscular junction of normal and P/Q Ca2+ channel knockout mouse. Neuroscience. 2004; 123(1): 75-85.
Alioua, A. Lu, R. Garcia-Valdes, J. Eghbali, M. Stefani, E. Toro, L. Diminished MaxiK channel expression alters 5-HT-induced contraction in aged rat aorta. Biophysical Journal 2004; 86(1): 121a-122a.
Ropero, A. B. Deva, R. Eghbali, M. Alioua, A. Toro, L. Stefani, E. Estrogen receptor alpha localizes in several subcellular compartments in adult rat cardiomyocytes. Biophysical Journal 2004; 86(1): 50a-51a.
Nishimaru, K. Eghbali, M. Stefani, E. Toro, L. Function and clustered expression of MaxiK channels in cerebral myocytes remain intact with aging. Experimental Gerontology. 2004; 39(5): 831-839.
Nishimaru, K. Eghbali, M. Rong, L. Marijic, J. Stefani, E. Toro, L. Functional and molecular evidence of MaxiK channel ?1 subunit decrease with coronary artery ageing in the rat. J Physiol. 2004; 559(3): 849-862.
Deva, R. Eghbali, M. Ropero, A. B. Toro, L. Stefani, E. Heart transcript levels of estrogen receptor alpha but not beta are under hormonal control. Biophysical Journal 2004; 86(1): 50a-50a.
Eghbali, M. Toro, L. Stefani, E. High resolution confocal microscopy and 3D deconvolution reveals that membrane and cytosolic proteins can be organized in distinct cluster patterns. Biophysical Journal 2004; 86(1): 446a-446a.
Eghbali, M. Toro, L. Stefani, E. MaxiK channel localizes in dense clusters at the surface of nonpregnant mouse myometrial cells which become undetectable in late pregnancy. Biophysical Journal 2004; 86(1): 521a-521a.
Toro, L. Alioua, A. Mahajan, A. Nishimaru, K. Zarei, M. M. Stefani, E. MaxiK, c-Src and vasoconstriction. J Muscle Res Cell Motil. 2004; 25(8): 616-7.
Stefani, E. Eghbali, M. Minosyan, T. Alioua, A. Toro, L. Molecular studies in heart hypertrophy during pregnancy. J Muscle Res Cell Motil. 2004; 25(8): 607.
Haug, T. Sigg, D. Ciani, S. Toro, L. Stefani, E. Olcese, R. Regulation of K+ flow by a ring of negative charges in the outer pore of BKCa channels. Part I: Aspartate 292 modulates K+ conduction by external surface charge effect. J Gen Physiol. 2004; 124(2): 173-84.
Haug, T. Olcese, R. Toro, L. Stefani, E. Regulation of K+ flow by a ring of negative charges in the outer pore of BKCa channels. Part II: Neutralization of aspartate 292 reduces long channel openings and gating current slow component. J Gen Physiol. 2004; 124(2): 185-97.
Tanaka, Y. Koike, K. Alioua, A. Shigenobu, K. Stefani, E. Toro, L. beta 1-subunit of MaxiK channel in smooth muscle: a key molecule which tunes muscle mechanical activity. Journal of Pharmacological Sciences. 2004; 94(4): 339-347.
Eghbali, M. Toro, L. Stefani, E. Diminished surface clustering and increased perinuclear accumulation of large conductance Ca(2+-)activated K+ channel in mouse myometrium with pregnancy. Journal of Biological Chemistry. 2003; 278(46): 45311-45317.
Sigg, D. Bezanilla, F. Stefani, E. Fast gating in the shaker K+ channel and the energy landscape of activation. Proceedings of the National Academy of Sciences of the United States of America. 2003; 100(13): 7611-7615.
Sartoris, S. Testi, M. G. Stefani, E. Chignola, R. Guerriero, C. Matucci, A. Cestari, T. Scarpa, A. Riviera, A. P. Zanoni, G. Tridente, G. Andrighetto, G. Induction of an antitumour adaptive immune response elicited by tumour cells expressing de novo B7-1 mainly depends on the anatomical site of their delivery: the dose applied regulates the expansion of the response. Immunology. 2003; 110(4): 474-81.
Madonini, E. Agostinis, F. Barra, R. Berra, A. Donadio, D. Pappacoda, A. Stefani, E. Tierno, E. Long-term and preventive effects of sublingual allergen-specific immunotherapy: a retrospective, multicentric study. Int J Immunopathol Pharmacol. 2003; 16(1): 73-9.
Guerriero, C. Zoccatelli, G. Stefani, E. Sartoris, S. Cestari, T. Riviera, A. P. Tridente, G. Andrighetto, G. Chignola, R. Myelin basic protein epitopes secreted by human T cells encounter natural autoantibodies in the serum. J Neuroimmunol. 2003; 141(1-2): 83-9.
Toro, L. Marijic, J. Nishimaru, K. Tanaka, Y. Song, M. Stefani, E. Aging, ion channel expression, and vascular function. Vascul Pharmacol. 2002; 38(1): 73-80.
Vella, A. Sartoris, S. Bambara, L. Ortolani, R. Carletto, A. Biasi, D. Stefani, E. Tridente, G. Cell contact-dependent PMN HLA-DR and CD69 membrane expression induced by autologous mono-lymphocytes and cell lines. Inflammation. 2002; 26(4): 143-52.
Alioua, A. Mahajan, A. Nishimaru, K. Zarei, M. M. Stefani, E. Toro, L. Coupling of c-Src to large conductance voltage- and Ca2+-activated K+ channels as a new mechanism of agonist-induced vasoconstriction. Proceedings of the National Academy of Sciences of the United States of America. 2002; 99(22): 14560-14565.
Eghbali, M. Olcese, R. Zarei, M. M. Toro, L. Stefani, E. External pore collapse as an inactivation mechanism for Kv4.3 K+ channels. Journal of Membrane Biology. 2002; 188(1): 73-86.
Helguera, G. Olcese, R. Song, M. Toro, L. Stefani, E. Tissue-specific regulation of Ca2+ channel protein expression by sex hormones. Biochimica Et Biophysica Acta-General Subjects. 2002; 1569(1-3): 59-66.
Olcese, R. Sigg, D. Latorre, R. Bezanilla, F. Stefani, E. A conducting state with properties of a slow inactivated state in a Shaker K+ channel mutant. Journal of General Physiology. 2001; 117(2): 149-163.
Zarei, M. M. Zhu, N. Alioua, A. Eghbali, M. Stefani, E. Toro, L. A novel MaxiK splice variant exhibits dominant-negative properties for surface expression. Journal of Biological Chemistry. 2001; 276(19): 16232-16239.
Zhang, Z. M. Tang, J. S. Tikunova, S. Johnson, J. D. Chen, Z. G. Qin, N. Dietrich, A. Stefani, E. Birnbaumer, L. Zhu, M. X. Activation of Trp3 by inositol 1,4,5-trisphosphate receptors through displacement of inhibitory calmodulin from a common binding domain. Proceedings of the National Academy of Sciences of the United States of America. 2001; 98(6): 3168-3173.
Marijic, J. Li, Q. X. Song, M. Nishimaru, K. Stefani, E. Toro, L. Decreased expression of voltage- and Ca2+-activated K+ channels in coronary smooth muscle during aging. Circulation Research. 2001; 88(2): 210-216.
Song, M. Helguera, G. Eghbali, M. Zhu, N. Zarei, M. M. Olcese, R. Toro, L. Stefani, E. Remodeling of Kv4.3 potassium channel gene expression under the control of sex hormones. Journal of Biological Chemistry. 2001; 276(34): 31883-31890.
Sigg, D. Bezanilla, F. Stefani, E. Temperature dependence of the early component of gating currents from Shaker K channels. Biophysical Journal 2001; 80(1): 219a-219a.
Olcese, R. Sigg, D. M. Latorre, R. Stefani, E. Bezanilla, F. A conducting slow inactivated Shaker channel?. Biophysical Journal 2000; 78(1): 214a-214a.
Li, Q. Marijic, J. Song, M. Stefani, E. Toro, L. Change of Matik channel density in coronary artery with aging. Biophysical Journal 2000; 78(1): 93a-93a.
Song, M. Zhu, N. Eghbali, M. Toro, L. Stefani, E. Changes of expression levels of Kv4.3 channels during pregnancy. Biophysical Journal 2000; 78(1): 449a-449a.
Zarei, M. M. Tora, L. Stefani, E. Characterization of Ca2+ channel subtypes in the supraoptic nucleus. Biophysical Journal 2000; 78(1): 199a-199a.
Stefani, E. Sigg, D. Bezanilla, F. Correlation between the early component of gating current and total gating current in shaker K channels. Biophysical Journal 2000; 78(1): 7A-7A.
Platano, D. Qin, N. Noceti, F. Birnbaumer, L. Stefani, E. Olcese, R. Expression of the alpha(2)delta subunit interferes with prepulse facilitation in cardiac L-type calcium channefs. Biophysical Journal. 2000; 78(6): 2959-2972.
Olcese, R. Toro, L. Stefani, E. Reduction of single channel conductance by D to N mutation in the hSlo potassium channel pore. Biophysical Journal 2000; 78(1): 93a-93a.
Song, M. Barila, B. Olcese, R. Zhu, N. Toro, L. Stefani, E. Regulation of MaxiK channel expression during pregnancy. Journal of Physiology-London 2000; 523(number): 51s-52s.
Madonini, E. Agostinis, F. Barra, R. Berra, A. Donadio, D. Stefani, E. Pappacoda, A. Tierno, E. Safety and efficacy evaluation of sublingual allergen-specific immunotherapy - A retrospective, multicenter study. International Journal of Immunopathology and Pharmacology. 2000; 13(2): 77-81.
Helguera, G. Song, M. Olcese, R. Toro, L. Stefani, E. Tissue specific changes in expression levels of Ca2+ and K+ channels during pregnancy. Biophysical Journal 2000; 78(1): 350a-350a.